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KMID : 1094720080130020123
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Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 2 p.123 ~ p.135
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High cell density ethanol fermentation in an upflow packed-bed cell recycle bioreactor
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Chang Ho-Nam
Kim Byoung-Jin
Kang Jong-Won
Jeong Chang-Moon
Kim Nag-Jong
Park Joong-Kon
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Abstract
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An upflow packed-bed cell recycle bioreactor (IUPCRB) is proposed for obtaining a high cell density. The system is comprised of a stirred tank bioreactor in which cells are retained partially by a packed-bed. A 1.3 cm (ID) × 48 cm long packed-bed was installed inside a 2 L bioreactor (working volume 1 L). Continuous ethanol fermentation was carried out using a 100 g/L glucose solution containing Saccharomyces cerevisiae (ATCC 24858). Cell retention characteristics were investigated by varying the void fraction (VF) of the packed bed by packing it with particles of 0.8∼2.0 mm sized stone, cut hollow fiber pieces, ceramic, and activated carbon particles. The best results were obtained using an activated carbon bed with a VF of 30∼35%. The IUPCRB yielded a maximum cell density of 87 g/L, an ethanol concentration of 42 g/L, and a productivity of 21 g/L/h when a 0.5 h?1 dilution rate was used. A natural bleeding of cells from the filter bed occurred intermittently. This cell loss consisted of an average of 5% of the cell concentration in the bioreactor when a high cell concentration (approximately 80 g/L) was being maintained.
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KEYWORD
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high cell density, void fraction, upflow packed-bed, activated carbon
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